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OCR was recorded three times under basal conditions and three times after the addition of Mito Stress compounds (Mito Stress Test Kit, Agilent, Santa Clara, California, USA): oligomycin (oligo, 1 M, adenosine triphosphate (ATP)-synthase inhibitor), fluorocarbonyl cyanide phenylhydrazone (FCCP, 0.5 M, decoupler), and rotenone/antimycin A (rot/AA, 0.5 M, complex 3/1 inhibitor), as shown in Figure 1

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The activity of folylpolyglutamate synthase is lowest towards 5-methyl-THF and this prevents this form of the vitamin from being trapped within intestinal enterocytes